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This study aimed to evaluate the differences in growth, development, and color of ‘Antique Curl’ cut rose flowers in response to different artificial light sources. Experiments were conducted in a glass greenhouse facility with an environment similar to that maintained by rose farmers, with a supply of nutrient solution and insecticides through a drip system while maintaining a temperature of 15 °C or higher. The growth was measured with the length and thickness of the rose stem and the assessment of photosynthesis and chlorophyll. Also, the color of the flowers was assessed using a spectrophotometer and visual analysis. The results of this study are as follows: First, the best possible results for the development and color of flowers, according to horticultural standards, were observed under an induction sodium lamp. Second, the difference in growth and development, depending on the type of artificial light source used, was less as compared to the difference in flower color. The flower color showed marked differences depending on the type of artificial light. This result shows that a variety of flowering plants can be produced using artificial light sources, and developing such variety in flower products could increase their economic value. However, it is necessary to consider other variables, such as the lifespan of roses, preferred colors, and various species per customer preferences, for future research.