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The study was conducted to investigate the effect of propolis on the increase of the immunity of honey-bee bees by gradually administering the bees’ propolis to bees. 0.16% and 0.3% 0.6% Propolis were administered at intervals of 2 weeks for 2 months, respectively, and 16 kinds of representative diseases of bees were tested. As a result, less bee disease was observed directly in the 0.3% propolis-ad-ministered group. In the next year, bees and a newborn bee showed a decrease in the incidence of bee disease and American foulbrood in bees administered with propolis. Based on the results of these stud-ies, it was confirmed that propolis administration in bees helps to enhance immunity of bees and is effective in controlling American foulbrood.