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Shrub growth and fruit characteristics and working time of ‘Duke’ blueberry by shrub distance were investigated. Overapping rate of blueberry crown was 109% in 0.6 m shrub distance, 37% in 1.2 m shrub distance, and -88% in 2.4 m shrub distance, respectively. The number of main stem tended to increase as the shrub spacing widened. The other fruit characteristics, except for the fruit weight, did not show any significantly different according to the shrub distance. The yield per 10 a was 2,097 kg for 0.6 m shrub distance, 1,303 kg for 1.2 m shrub distance, and 710.7 kg for 2.4 m shrub distance in 2019, respectively. Total working time was 154 hr for 0.6 m shrub distance, 114 hr for 1.2 m shrub distance, and 74 hr for 2.4 m shrub distance. Therefore, the blueberry shrub distance was judged to be appropriate about 1.2 m in the long-termly. However, in order to increase yield early and income, the shrub can maintain 0.6 m distance during young shrub, it can managed flexibly to 1.2 m distance when it becomes a mature shrub.