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The purpose of this study was to assess the influence of the robo-horseback riding exercise with riding posture and speeds on muscle activity of neck and upper trunk in health adults. Subjects(n=31) performed robo-horseback riding with three different kind of postures(hyper-lordotic posture: HLP, neutral posture: NP, slumper posture: SP) and two kind of speed conditions(moderate speed: MD, fast speed: FS). Muscle activity of neck and upper trunk(sternocleidomastoid: SCM, upper trapezius: U-Trap, cervical elector spinae: CES, thoracic elector spinae: TES, lumbar elector spinae: LES, internal oblique: IO, rectus abdominis: RA) were recorded during the robo-horseback riding. According to this study, main effect of muscle activity with SCM, TES, and IO depended on the riding posture(p<.05). The muscle activity of SCM and IO in HLP were significantly lower and SCM and TES in SP were significantly higher than NP(p<.05), respectively. Additionally, The main effect with muscle activity of LES, IO, and RA was influenced by speed(p<.05). The muscle activity of LES, IO, and RA were significantly increase depended on the changed speed from moderate to fast(p<.05)<Table 3>. Therefore, neutral posture was especially important for healthy robo-horseback riding.