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Purpose: To evaluate the effects of ocutome diameter and cutting rate on the yield of diagnostic vitrectomy. Methods: Blood samples were obtained from a 40-year-old healthy male and treated with ethylenediaminetetraacetic acid. Samples were aspirated under cutting rates of 0, 800, 1,200, 2,500, 5,000, and 8,000 cuts per minute (CPM) using a 25- or 27-gauge vitrectomy cutter. The white blood cell (WBC) break rate and hemolysis index were calculated to evaluate the quality of the specimen. Results: A WBC count of 0-1.82% was unidentifiable in the collected blood using the 25-gauge ocutome, as was that of 0.87-8.00% under a 27-gauge ocutome. The ratio of unidentifiable WBCs increased with increasing cutting rate (p = 0.030). This ratio did not show any difference depending on diameter of the vitrectomy cutter (p = 0.763). The hemolysis index increased with the 25-gauge cutter compared to the 27-gauge. Both ocutome diameter and cutting rate affected the hemolysis index (p < 0.001, p = 0.033). Conclusions: The results of the current study using blood samples showed that the small-gauge ocutome increased only the hemolysis index, while both the hemolysis index and the WBC break rate were higher at higher cutting rates. These results suggest that, when collecting a vitreous specimen by diagnostic vitrectomy, the ocutome diameter is not important. However, when collecting vitreous specimens at high CPM, attention is required because the hemolysis index and WBC break rate increase.
