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In this study, we determined the effect of different concentrations of Cd on the growth of Pleurotus ostreatus mycelia, which was confirmed using PDA, PDB, and a column test. The efficacy of treatment was evaluated using Cd at two concentrations, 10 ppm and 100 ppm. The extent of mycelial growth on PDA and PDB was similar to that observed in the untreated groups and those treated with 10 ppm Cd. However, it was found that the mycelial growth was suppressed in a system treated with Cd at concentrations of 100 ppm. Moreover, the extent of mycelial growth observed upon conducting a column test was similar to that obtained using PDA and PDB. When the composition of free amino acids in PDB was determined, their levels in the group treated with 100 ppm Cd were found to be similar to those of the control. However, the amounts of 15 amino acids in the group treated with 10 ppm of Cd had increased compared to those detected in the control.
