초록 열기/닫기 버튼
목적: 본 연구는 인간각질형성세포(HaCaT)에서 자외선(UV) 손상에 대한 항산화 및 항염증 효과를 확인하여 피부노화 예방 화장품 소재로서 sinapic acid의 가능성을 확인하고자 한다. 방법: Sinapic acid를 농도별로 HaCaT에 처리 후 UVB로 산화적 스트레스를 유도하였다. UVB에 대한 sinapic acid의 세포보호효과를 확인하기 위하여 water-soluble tetrazolium salt (WST-1)를 이용하였으 며, UVB에 의하여 생성된 활성산소종(reactive oxygen species, ROS)의 농도변화는 2’,7’-dichlorofluorescin diacetate (DCF-DA)를 사용하여 측정하였다. 항산화 및 염증과 관련된 유전자 발현은 quantitative real-time polymerase chain reaction (qRT-PCR)을 통해 확인하였다. 결과: Sinapic acid는 UVB에 의한 세포독성으로부터 세포를 보호하고, ROS의 생성을 감소시킨다. 항산화와 관련한 유전자인 superoxide dismutase 1 (SOD1), catalase (CAT), glutathione peroxidase 1 (GPX1), nuclear factor (erythroid-derived 2)-like 2 (NRF2) 및 heme oxygenase 1 (HO1)의 발현을 증가하게 하였고, 지질 과산화는 농도 의존적으로 감소하였다. 또한, 염증과 관련이 있는 cyclooxygenase 2 (COX2), interleukin 6 (IL6)와 interleukin 8 (IL8)의 유전자 발현은 농도 의존적으로 감소하였다. 결론: 본 연구를 통하여 sinapic acid가 UVB에 대하여 항산화 및 항염증의 효과가 있음을 확인하였으며 이는 노화예방 화장품 성분 으로서의 가능성이 있을 것으로 사료된다.
Purpose: The purpose of the present study was to explore the possibility of using sinapic acid as a skin anti-aging cosmetic by demonstrating its antioxidant and anti-inflammatory effects in keratinocytes damaged by ultraviolet (UV) irradiation. Methods: The effects of sinapic acid against oxidative stress were examined in a UVB-induced aging model of human keratinocyte HaCaT cells. The water-soluble tetrazolium salt (WST-1) assay was employed to examine protective effects against UVB. Changes in the concentration of reactive oxygen species (ROS) produced by UVB were measured using 2’,7’-dichlorofluorescin diacetate (DCF-DA). Expression of genes associated with anti-oxidant and anti-inflammatory effects were confirmed using quantitative real-time polymerase chain reaction (qRT-PCR). Results: Sinapic acid protected cells from UVB-induced cytotoxicity and reduced ROS produced by UVB irradiation. Expression of genes associated with antioxidant effects including, superoxide dismutase 1 (SOD1), catalase (CAT), glutathione peroxidase 1 (GPX1), nuclear factor (erythroid-derived 2)-like 2 (NRF2), and heme oxygenase 1 (HO1), increased and lipid peroxidation decreased in a dose-dependent manner. The expression of cyclooxygenase 2 (COX2), interleukin 6 (IL6), and interleukin 8 (IL8) genes, which are associated with inflammation, also decreased in a dose-dependent manner. Conclusion: These results suggest that sinapic acid has potential as an anti-aging cosmetic ingredient with anti-oxidant, anti-inflammatory properties against UVB irradiation.
目的: 探讨芥子酸对UVB诱导损伤的人角质形成细胞的抗氧化以及抗炎功效，确认芥子酸作为抗衰老化妆品原料的可 行性。方法: 对HaCaT细胞用不同浓度的芥子酸处理后，利用UVB进行氧化应激。为确认芥子酸的细胞保护作用，利用 water-soluble tetrazolium salt (WST-1)方法来评价；为确认由UVB诱导产生的活性氧 (reactive oxygen species, ROS)的 浓度变化，利用2’,7’-dichlorofluorescin diacetate (DCF-DA)来进行测定；为确认与抗氧化和抗炎相关的遗传因子的表达 程度，利用quantitative real-time polymerase chain reaction (qRT-PCR)方法来测定。结果: 芥子酸由UVB产生的细胞 毒性中保护细胞，并减少ROS的生成。芥子酸增加与抗氧化相关的遗传因子superoxide dismutase 1 (SOD1)、catalase (CAT)、glutathione peroxidase 1 (GPX1)、nuclear factor (erythroid-derived 2)-like 2 (NRF2)以及heme oxygenase 1 (HO1)等的表达程度，并以浓度依赖性方式减少脂质过氧化。此外，与炎症相关的遗传因子cyclooxygenase 2 (COX2)、 interleukin 6 (IL6)以及interleukin 8 (IL8)的表达以浓度依赖性方式减少。结论: 从以上的研究结果，芥子酸对UVB具有抗 氧化和抗炎作用，因此作为预防衰老的化妆品原料充分具有可行性。
키워드열기/닫기 버튼
Sinapic acid, Anti-oxidant, Anti-inflammation, UVB, HaCaT
芥子酸, 抗氧化, 抗炎, UVB, 人角质形成细胞
