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Purpose: The purpose of this study was to identify the influential factors on job satisfaction among nursing assistants working in medical institutions. Methods: Small and medium-sized hospitals and general hospitals (S City, U City, N City), 11 Geriatric hospitals (S City, N City, U City, T City, I City), and 29 clinics (S City, I City, N City). Data was collected from 194 employed nursing assistants from February 15 to April 15, 2021. The obtained data were analyzed using descriptive statistics, ANOVA, Scheffe's test, Pearson's correlation analysis, and hierarchical regression analysis. Results: We confirmed a positive correlation between job satisfaction and organizational commitment, and conducted a hierarchical multiple regression analysis using monthly income, work-life balance, organizational commitment, and work confidence as influencing factors for job satisfaction, and found that organizational commitment and work confidence were significant influencing factors for job satisfaction. Conclusion: To improve the job satisfaction of nursing assistants, it is necessary to improve their understanding of the organizational culture in the workplace and expand education and training opportunities to enhance their work confidence to induce long-term retention. In addition, it was suggested that clear job scope and adequate compensation are necessary for efficient human resource management.